The attack occurred in central Mali as the soldiers from Togo, part of the U.N. Multidimensional Integrated Stabilization Mission in Mali (MINUSMA), were travelling in a convoy around 30 kilometers west of Sevare.
No group has yet claimed responsibility for the attack.
“The Secretary-General presents his heartfelt condolences to the families of the five peacekeepers who have died in the cause of peace and to the government and people of Togo,” a U.N. statement said.
A similar ambush Friday killed five Malian soldiers and wounded four more when their vehicle hit a mine in the north and they were then shot at by unknown assailants.
After Sunday’s attack, the number of U.N. peacekeepers killed while on active service in Mali rose to at least 64, making it the most dangerous active deployment for the U.N. in the world.