The five men pleaded not guilty to 152 counts of committing acts of terrorism in a Nairobi court on Thursday.
Four al-Shabab gunmen stormed the Garissa University College at dawn on April 2. The attack went on for hours before it was finally ended by a specialised police unit and the four men were killed.
The prosecution alleges that the five suspects colluded to carry out the attack, but have not said what their roles were.
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Speaking through an interpreter, the four Somalis and a Tanzanian claimed their heads were forced into buckets full of water, and that they were electrocuted and whipped after they denied involvement in the attack.
Al-Shabab has vowed to carry out attacks against Kenya for sending its troops to Somalia to fight the armed group.
Kenya has experienced a wave of explosive and gun attacks since October 2011 when its troops went into Somalia.
Source: AP